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Immunology and Infection

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JoVE Journal Immunology and Infection
Kwantitatieve PCR van T7 bacteriofaag van Biopanning
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Kwantitatieve PCR van T7 bacteriofaag van Biopanning

Article DOI: 10.3791/58165-v • 05:42 min • September 27th, 2018
September 27th, 2018 •
1, 1, 1, 1, 1
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy, University of Texas at Austin

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Een reproduceerbare, accuraat en tijd efficiënt kwantitatieve PCR (qPCR) methode om op te sommen T7 bacteriofaag wordt hier beschreven. Het protocol geeft een duidelijke beschrijving van phage genomic DNA voorbereiding, PCR reactie voorbereiding, qPCR fietsen voorwaarden en qPCR data-analyse.

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Immunologie en infecties probleem 139 T7 phage faag genomic DNA qPCR plaque assay aantoonbaarheidsgrens kwantificering bereik
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