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微穿刺2光子显微镜辅助小鼠鲍曼空间的研究
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Micropuncture of Bowman’s Space in Mice Facilitated by 2 Photon Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
微穿刺2光子显微镜辅助小鼠鲍曼空间的研究
DOI:
10.3791/58206-v
•
07:37 min
•
October 11, 2018
•
Katsuyuki Matsushita
,
Kirsti Golgotiu
,
Daniel J. Orton
,
Richard D. Smith
,
Karin D. Rodland
,
Paul D. Piehowski
,
Michael P. Hutchens
3
1
Anesthesiology & Perioperative Medicine
,
Oregon Health & Science University
,
2
Environmental and Biological Services Division
,
Pacific Northwest National Laboratory
,
3
Operative Care Division
,
Portland Veterans Affairs Medical Center
Chapters
00:04
Title
00:42
Kidney Stabilization and Injection
02:35
Glomerulus Selection and Bowman s Space Fluid Aspiration
05:41
Results: Representative Bowman s Space Micropuncture and Analysis
07:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们目前使用2光子显微术在鲍曼的小鼠泌尿空间内放置一个微量吸管, 结合2个肾脏生理学的基础技术。使用2光子显微术克服了传统显微术微穿刺肾脏生理学研究的关键局限性。
Tags
Micropuncture
Bowman’s Space
Two-photon Microscopy
Single-nephron Physiology
Glomerular Filtration
Tubular Physiology
Mouse Preparation
Kidney Stabilization
FITC-Dextran
Target Glomerulus
Pipette Positioning
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