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JoVE Journal
Biochemistry

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基于寡核苷酸的串联 RNA 分离法恢复真核 mRNA-蛋白复合物
 

基于寡核苷酸的串联 RNA 分离法恢复真核 mRNA-蛋白复合物

Article doi: 10.3791/58223
August 18th, 2018

Summary August 18th, 2018

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本文介绍了一种用于恢复内在形成的 mRNA 蛋白复合物的串联 RNA 分离过程 (跳闸)。具体来说, rna 蛋白复合物在体内交联, polyadenylated rna 与寡核苷酸 (dT) 珠提取物分离, 特殊基因用修饰 RNA 反义寡核苷酸捕获。通过应用免疫印迹分析, 检测到基因的蛋白质。

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