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高通量荧光素酶法评价单周转蛋白酶 PCSK9 的蛋白质水解
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A High-Throughput Luciferase Assay to Evaluate Proteolysis of the Single-Turnover Protease PCSK9
DOI:

08:14 min

August 28, 2018

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Chapters

  • 00:04Title
  • 00:43High-throughput Luciferase-based Proteolysis Assay
  • 02:50Transfection (Day 1)
  • 04:32Assay (Day 2)
  • 06:41Results: Alterations in PCSK9 Proteolytic Activity are Common in Clinical Populations
  • 07:52Conclusion

Summary

Automatic Translation

本协议提出了一种评估细胞内一种内在的低活性、单周转蛋白酶的蛋白水解活性的方法。具体来说, 该方法用于评价 PCSK9 的蛋白水解活性, 这是脂代谢的关键驱动力, 其蛋白水解活性是其最终胆固醇功能所必需的。

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