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08:52 min
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July 28, 2018
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Summary
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På grund av den höga fetthalten, har fettvävnad utmanande för att visualisera med traditionella histologiska metoder. Adipo-Clear är en vävnad som clearing teknik som gör robusta märkning och högupplösta volymetriska fluorescerande avbildning av fettvävnad. Här, beskriver vi metoderna för provberedning, förbehandling, färgning, clearing och montering för avbildning.
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Cite this Article
Chi, J., Crane, A., Wu, Z., Cohen, P. Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue. J. Vis. Exp. (137), e58271, doi:10.3791/58271 (2018).
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