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Biology

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Laser-assisted Lentiviral gen leverans till mus befruktade ägg
 
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Laser-assisted Lentiviral gen leverans till mus befruktade ägg

Article DOI: 10.3791/58327
November 1st, 2018

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Summary November 1st, 2018

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Click here for the English version.

Mus befruktade ägg och tidiga skede embryon skyddas av zona pellucida, ett glykoprotein matris som bildar en barriär mot gen leverans. I artikeln beskrivs ett protokoll för perforering av zona med laser transduce embryonala celler med lentiviral vektorer och skapa transgena möss.

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