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Hvordan man kan kvantificere brøkdel af Photoactivated fluorescerende proteiner i Bulk og i levende celler
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Chapters
- 00:04Title
- 01:20Cell Culture and Transfection
- 02:31Imaging and Photoactivation
- 05:45Imaging Analysis and Algorithm for Ratiometric Intensity-based Quantification of Photoactivation Efficiency
- 09:09Results: Maximizing Photoactivation Efficiency
- 09:50Conclusion
Vi præsenterer her, en protokol, der involverer genetisk koblede spektralt særskilte photoactivatable og fluorescerende proteiner. Disse fluorescerende proteiner kimærer tillade kvantificering af de PA-FP fraktion, der er photoactivated at være fluorescerende, dvs., photoactivation effektivitet. Protokollen afslører, at forskellige former for photoactivation give forskellige photoactivation effektivitet.
