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Hvordan man kan kvantificere brøkdel af Photoactivated fluorescerende proteiner i Bulk og i levende celler
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
DOI:

11:03 min

January 07, 2019

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Chapters

  • 00:04Title
  • 01:20Cell Culture and Transfection
  • 02:31Imaging and Photoactivation
  • 05:45Imaging Analysis and Algorithm for Ratiometric Intensity-based Quantification of Photoactivation Efficiency
  • 09:09Results: Maximizing Photoactivation Efficiency
  • 09:50Conclusion

Summary

Automatic Translation

Vi præsenterer her, en protokol, der involverer genetisk koblede spektralt særskilte photoactivatable og fluorescerende proteiner. Disse fluorescerende proteiner kimærer tillade kvantificering af de PA-FP fraktion, der er photoactivated at være fluorescerende, dvs., photoactivation effektivitet. Protokollen afslører, at forskellige former for photoactivation give forskellige photoactivation effektivitet.

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