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补充蛋白C1q和透明质酸在促进细胞粘附中的相互作用评价
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Immunology and Infection
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JoVE Journal Immunology and Infection
Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
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补充蛋白C1q和透明质酸在促进细胞粘附中的相互作用评价

DOI:
10.3791/58688-v

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06:54 min

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June 15, 2019

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, , , , , , , ,
1Department of Life Sciences,University of Trieste, 2Institute for Maternal and Child Health,IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, 3Department of Medical, Surgical and Health Science,University of Trieste

Chapters

  • 00:00Title
  • 00:59HA and C1q Coating Procedure
  • 02:45Cells Labelling with Fast Dil
  • 04:04Cells Adhesion on HA/C1q Matrices
  • 05:32Results
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

补体组分C1q是一种在组织微环境中高度表达的亲炎分子,可与细胞外基质相互作用。在这里,我们描述了一种测试C1q与透明质酸结合如何影响细胞粘附的方法。

Tags

C1qHyaluronic AcidCell AdhesionExtracellular MatrixInnate Immune SystemTumor MicroenvironmentPlacenta MicroenvironmentCell DivisionBinary CellsCoating ProcedureEnzyme-linked ImmunoassayFast DiICell Labeling

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