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Journal / Neuroscience / Encellede RNA sekvensering av Fluorescently…
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Neuroscience

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JoVE Journal Neuroscience
Encellede RNA sekvensering av Fluorescently merket musen Neurons med manuell sortering og dobbel In Vitro transkripsjon absolutte teller sekvensering (DIVA-Seq)
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Encellede RNA sekvensering av Fluorescently merket musen Neurons med manuell sortering og dobbel In Vitro transkripsjon absolutte teller sekvensering (DIVA-Seq)

Article DOI: 10.3791/58690-v • 07:49 min • October 26th, 2018
October 26th, 2018 •
1, 1
1Cold Spring Harbor Laboratory

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Denne protokollen beskriver manuell sortering fremgangsmåten for å isolere enkelt fluorescently merket neurons etterfulgt av i vitro transkripsjon-baserte mRNA forsterkning for høy-dybde encellede RNA sekvensering.

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Nevrovitenskap problemet 140 manuell sortering Nevron encellede RNA sekvensering DIVA-Seq i vitro transkripsjon lineær forsterkning unik molekylær identifikator (UMI)
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