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荧光标记小鼠神经元的单细胞 RNA 序列的人工分选和双体外转录与绝对计数测序 (天后-Seq)
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Neuroscience
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JoVE Journal Neuroscience
Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)
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荧光标记小鼠神经元的单细胞 RNA 序列的人工分选和双体外转录与绝对计数测序 (天后-Seq)

DOI:
10.3791/58690-v

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07:49 min

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October 26, 2018

•

,
1Cold Spring Harbor Laboratory

Chapters

  • 00:04Title
  • 00:43Preparation for Dissection and Neuron Sorting
  • 02:09Brain Sectioning and Cell Dissociation
  • 04:15Manual Sorting of Fluorescently Labeled Mouse Neurons
  • 05:59Results: Microdissection of GABAergic Neurons
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了将单个荧光标记神经元与体外转录的 mRNA 扩增分离以进行高深度单细胞 RNA 测序的人工分拣程序。

Tags

Single-cell RNA SequencingFluorescently Labeled NeuronsManual SortingDouble In Vitro TranscriptionDIVA-SeqNeuron IsolationSubpopulationFACS SortingGlass MicrocapillariesArtificial Cerebrospinal FluidProteaseFBSPasteur PipettesBrain DissectionVibratomeLabeled CellsActivity Blockers

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