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hiv 转录和拼接中延迟逆转剂的高通量体外评价
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
DOI:

07:18 min

January 22, 2019

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Chapters

  • 00:04Title
  • 00:32Transfection of HEK293T Cells with Dual Color Reporter Construct
  • 02:01Treatment of Transfected HEK293T Cells with Latency Reversing Agents
  • 02:33Staining of Transfected Cells with Fixable Viability Dye for Flow Cytometry Analysis
  • 04:40EGFP and DsRed Measurements by Flow Cytometry and Data Analysis
  • 05:49Results: Assessment of Latency Reversing Agents on HIV Transcription and Splicing
  • 06:43Conclusion

Summary

Automatic Translation

通过测试干预措施对艾滋病毒转录和剪接的影响, 描述并应用了一种用于艾滋病毒高效再激活和清除的高通量协议。给出了延迟反转剂对 ltr 驱动的转录和剪接的影响的代表性结果。

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