JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
hiv 转录和拼接中延迟逆转剂的高通量体外评价
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
Please note that all translations are automatically generated.
Click here for the English version.
hiv 转录和拼接中延迟逆转剂的高通量体外评价
DOI:
10.3791/58753-v
•
07:18 min
•
January 22, 2019
•
Georges Khoury
,
Damian F.J. Purcell
1
Department of Microbiology and Immunology
,
University of Melbourne
Chapters
00:04
Title
00:32
Transfection of HEK293T Cells with Dual Color Reporter Construct
02:01
Treatment of Transfected HEK293T Cells with Latency Reversing Agents
02:33
Staining of Transfected Cells with Fixable Viability Dye for Flow Cytometry Analysis
04:40
EGFP and DsRed Measurements by Flow Cytometry and Data Analysis
05:49
Results: Assessment of Latency Reversing Agents on HIV Transcription and Splicing
06:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
通过测试干预措施对艾滋病毒转录和剪接的影响, 描述并应用了一种用于艾滋病毒高效再激活和清除的高通量协议。给出了延迟反转剂对 ltr 驱动的转录和剪接的影响的代表性结果。
Tags
HIV
Latency Reversing Agents
Transcription
Splicing
In Vitro Assessment
High Throughput
Reporter Construct
Transfection
Cell Culture
Flow Cytometry
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
V3的HIV - 1取向的基因型推理使用人口为基础的测序
使用低温电子断层扫描和自动子断层扫描平均HIV包膜糖蛋白分子结构的测定
在体外
对HIV - 1核心脱壳
艾滋病毒的成像1信封引起的病毒学突触信号合成脂双层
在体外试验
,以评估免疫调节途径对HIV特异性CD4 + T细胞效应功能的影响
HIV逆转录酶和整合酶抑制剂的快速筛选
高通量荧光技术巨噬细胞吞噬功能和肌动蛋白聚合的评估
胞外DNA释放的高通量测量和人中性粒定量NET形成<em>体外</em
高通量基因定位在<em>布氏锥虫</em
测量<em>体外</em> HIV-1 Preintegration配合物的整合活动
Read Article