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Immunology and Infection
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近一代测序鉴定小鼠与人体抗体的分泌
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
近一代测序鉴定小鼠与人体抗体的分泌
DOI:
10.3791/58804-v
•
08:51 min
•
March 15, 2019
•
Lin Sun
,
Naoko Kono
,
Hiroyuki Toh
,
Hanbing Xue
,
Kaori Sano
5
,
Tadaki Suzuki
,
Akira Ainai
,
Yasuko Orba
,
Junya Yamagishi
8
,
Hideki Hasegawa
5
,
Yoshimasa Takahashi
,
Shigeyuki Itamura
,
Kazuo Ohnishi
10
1
Graduate School of Life and Environmental Sciences
,
University of Tsukuba
,
2
Center for Influenza Virus Research
,
National Institute of Infectious Diseases
,
3
School of Science and Technology
,
Kwansei Gakuin University
,
4
Department of Pathology
,
National Institute of Infectious Diseases
,
5
Division of Infectious Diseases Pathology, Department of Global Infectious Diseases
,
Tohoku University Graduate School of Medicine
,
6
Division of Molecular Pathobiology, Research Center for Zoonosis Control
,
Hokkaido University
,
7
Division of Collaboration and Education, Research Center for Zoonosis Control
,
Hokkaido University
,
8
Global Station for Zoonosis Control, GI-CoRE
,
Hokkaido University
,
9
Department of Immunology
,
National Institute of Infectious Diseases
,
10
Faculty of Life and Environmental Sciences
,
University of Tsukuba
Chapters
00:04
Title
01:23
Nucleic Acid Isolation from Immune Cells and Tissues
03:04
cDNA Synthesis and PCR Amplification
04:58
NGS Sequencing of Libraries
05:33
Results: Identification of Mouse and Human Antibody Repertoires
08:11
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 我们描述了分析和可视化整个抗体再灌注物质的结构和组成的协议。这包括使用下一代测序获取大量抗体 RNA 序列。
Tags
Antibody Repertoire
Next-generation Sequencing
B Cell Repertoire
Immune Response
Vaccine Efficacy
Immunoglobulin Amplification
RNA Extraction
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