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Journal
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Biochemistry
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通过肽浓缩和质谱检测蛋白质泛化位点检测
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Biochemistry
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JoVE Journal
Biochemistry
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
通过肽浓缩和质谱检测蛋白质泛化位点检测
DOI:
10.3791/59079-v
•
11:54 min
•
March 23, 2020
•
Karel Bezstarosti
,
Lennart van der Wal
,
Jeroen A. A. Demmers
1
Proteomics Center
,
Erasmus University Medical Center
Chapters
00:05
Introduction
01:16
Sample Preparation
03:36
Offline Peptide Fractionation
06:16
Nanoflow LC-MS/MS
07:51
Data Analysis
09:00
Results: Detection of diGly Peptides Using Nanoflow LC-MS/MS
10:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种从复杂生物样本中泛化的蛋白质中产生的二Gly肽的纯化、检测和鉴定方法。在泛源分析的深度级别方面,该方法具有可重复性、健壮性,优于已发布的方法。
Tags
Protein Ubiquitination
DiGly Peptides
Mass Spectrometry
SILAC
Protein Enrichment
Lys-C
Trypsin Digestion
TFA Precipitation
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