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Biology
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中通量筛选法评估对精子中 ca
2 +
信号和反应的影响
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JoVE Journal
Biology
Medium-throughput Screening Assays for Assessment of Effects on Ca
2+
-Signaling and Acrosome Reaction in Human Sperm
Please note that all translations are automatically generated.
Click here for the English version.
中通量筛选法评估对精子中 ca
2 +
信号和反应的影响
DOI:
10.3791/59212-v
•
05:44 min
•
March 01, 2019
•
Anders Rehfeld
2
,
Dorte Louise Egeberg Palme
2
,
Kristian Almstrup
2
,
Anders Juul
2
,
Niels Erik Skakkebaek
2
1
Department of Growth and Reproduction
,
Copenhagen University Hospital
,
2
International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC)
,
University of Copenhagen
Chapters
00:04
Title
00:40
Motile Sperm Cell Purification
01:28
Sperm Cell Counting
02:14
Calcium-fluorimetry
03:30
Acrosome Reaction Measurement
04:14
Results: Representative Calcium-signaling and Acrosome Reaction Analyses
05:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里, 描述了两个中等吞吐量检测评估对 ca2
+
信号和顶体反应的影响在人类精子中。这些检测方法可用于快速、轻松地筛选大量化合物, 以确定对人类精子中 ca
2 +
信号和顶体反应的影响。
Tags
Medium-throughput Screening
Calcium Signaling
Acrosome Reaction
Human Sperm
Sperm Cell Function
Compound Testing
HTF Medium
Sperm Cell Motility
Sperm Cell Concentration
Fluorescence Plate Reader
Calcium Concentration
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