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中通量筛选法评估对精子中 ca^{2 +}信号和反应的影响

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JoVE Journal Biology

Medium-throughput Screening Assays for Assessment of Effects on Ca²⁺-Signaling and Acrosome Reaction in Human Sperm

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中通量筛选法评估对精子中 ca^{2 +}信号和反应的影响

DOI:

10.3791/59212-v

•

05:44 min

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March 01, 2019

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Anders Rehfeld², Dorte Louise Egeberg Palme², Kristian Almstrup², Anders Juul², Niels Erik Skakkebaek²

¹Department of Growth and Reproduction,Copenhagen University Hospital, ²International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC),University of Copenhagen

Chapters

00:04Title
00:40Motile Sperm Cell Purification
01:28Sperm Cell Counting
02:14Calcium-fluorimetry
03:30Acrosome Reaction Measurement
04:14Results: Representative Calcium-signaling and Acrosome Reaction Analyses
05:01Conclusion

Summary

Automatic Translation

在这里, 描述了两个中等吞吐量检测评估对 ca2⁺信号和顶体反应的影响在人类精子中。这些检测方法可用于快速、轻松地筛选大量化合物, 以确定对人类精子中 ca^{2 +}信号和顶体反应的影响。

Tags

Keywords: Medium-throughput Screening Calcium Signaling Acrosome Reaction Human Sperm Sperm Cell Function Compound Testing HTF Medium Sperm Cell Motility Sperm Cell Concentration Fluorescence Plate Reader Calcium Concentration

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