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蓝色原代聚丙烯酰胺凝胶电泳免疫印迹法诱导人细胞线粒体呼吸链配合物的分析
JoVE Journal
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JoVE Journal
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Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
Please note that all translations are automatically generated.
Click here for the English version.
蓝色原代聚丙烯酰胺凝胶电泳免疫印迹法诱导人细胞线粒体呼吸链配合物的分析
DOI:
10.3791/59269-v
•
07:55 min
•
February 12, 2019
•
Svetlana Konovalova
1
Research Programs Unit, Molecular Neurology
,
University of Helsinki
Chapters
00:04
Title
00:25
Preparation of Mitochondrial Lysates
02:40
Preparation of Gradient Gel for BN-PAGE
05:32
Blue Native Gel Electrophoresis
06:40
Results: Investigating the Assembly of Mitochondrial OXPHOS Complexes Using BN-PAGE
07:16
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案通过蓝色原生聚丙烯酰胺凝胶电泳对线粒体呼吸链配合物进行了分析。这里介绍了应用于培养的人体细胞的方法。
Tags
Mitochondrial
Respiratory Chain
Complexes
Cultured
Human
Cells
Blue Native
Polyacrylamide
Gel
Electrophoresis
Immunoblotting
Oxidative
Phosphorylation
System
In-tact
Complexes
Assembly
PBS
Digitonin
Protease
Inhibitor
Mitochondrial
Buffer
Loreal
Mount
Gradient
Gel
PAGE
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