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蓝色原代聚丙烯酰胺凝胶电泳免疫印迹法诱导人细胞线粒体呼吸链配合物的分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
DOI:

07:55 min

February 12, 2019

Chapters

  • 00:04Title
  • 00:25Preparation of Mitochondrial Lysates
  • 02:40Preparation of Gradient Gel for BN-PAGE
  • 05:32Blue Native Gel Electrophoresis
  • 06:40Results: Investigating the Assembly of Mitochondrial OXPHOS Complexes Using BN-PAGE
  • 07:16Conclusion

Summary

Automatic Translation

该方案通过蓝色原生聚丙烯酰胺凝胶电泳对线粒体呼吸链配合物进行了分析。这里介绍了应用于培养的人体细胞的方法。

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