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线虫的耗氧率测定
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JoVE Journal Biology
Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
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线虫的耗氧率测定

DOI:
10.3791/59277-v

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08:10 min

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February 23, 2019

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,
1Department of Regenerative and Cancer Cell Biology,Albany Medical College

Chapters

  • 00:04Title
  • 00:32Growth and Synchronization of Nematode Population
  • 02:40Hydration of Probes, Drug Loading and Cartridge Equilibration
  • 04:40Preparation of Worm Plate, Assay Run, and Post-experiment Data Analysis
  • 06:45Results: OCR in Wild Type and Various sel-12 Mutants
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

线粒体呼吸对组织的生存至关重要;因此, 耗氧率是线粒体健康的一个很好的指标。在这个协议中, 我们描述了使用一个商业上可用的呼吸计来测量基本和最大耗氧率的活, 完整, 和自由运动的。

Tags

Keywords: Oxygen Consumption RateCaenorhabditis ElegansMitochondrial FunctionOxygen Consumption AssayC. ElegansL4 LarvaeOP50NGM PlatesFUdRRespirometrySynchronization

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