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Chapters
- 00:04Title
- 00:32Growth and Synchronization of Nematode Population
- 02:40Hydration of Probes, Drug Loading and Cartridge Equilibration
- 04:40Preparation of Worm Plate, Assay Run, and Post-experiment Data Analysis
- 06:45Results: OCR in Wild Type and Various sel-12 Mutants
- 07:36Conclusion
Mitokondrie respiration er kritisk for kropsligt overlevelse; ilt forbrugssats er derfor en udmærket indikator for mitokondrie sundhed. I denne protokol, vi beskriver brugen af en kommercielt tilgængelig respirometer at måle basal og maksimal ilt forbrug satser i live, intakt og frit motile Caenorhabditis elegans.
