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절연, 전파, 그리고 인간의 치과 펄프의 줄기 세포의 신경 분화 동안 프리온 단백질 표정
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
DOI:

11:50 min

March 18, 2019

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Chapters

  • 00:04Title
  • 00:47Tooth and Dental Pulp Extraction
  • 01:29Processing of the Dental Pulp and Stem Cell Release
  • 02:29Stem Cell Culture and Propagation
  • 03:44Transient PrPC Silencing by siRNA
  • 05:04Neuronal Induction Process of hDPSCs
  • 05:50Characterization of hDPSCs by Flow Cytometry
  • 08:03Evaluation of PrPC Expression in hDPSCs by Flow Cytometry Analysis
  • 09:14Results: hDPSCs Isolation and Propagation and the Role of PrP<supC During Neuronal Differentiation
  • 10:54Conclusion

Summary

Automatic Translation

여기 우리는 프리온 단백질 표정 신경 분화 과정을 평가 하기 위해 인간의 치과 펄프의 줄기 세포 분리 및 전파에 대 한 프로토콜을 제시.

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