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Biology
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利用成熟的细胞隔离试剂盒快速分离脂滴器
JoVE Journal
Biology
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JoVE Journal
Biology
Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit
Please note that all translations are automatically generated.
Click here for the English version.
利用成熟的细胞隔离试剂盒快速分离脂滴器
DOI:
10.3791/59290-v
•
08:43 min
•
April 19, 2019
•
Jascha Brettschneider*
1
,
Jason M. Correnti*
1
,
Chelsea Lin
,
Bianca Williams
,
Amanke Oranu
,
Amy Kuriakose
,
Dru McIver-Jenkins
,
Abigail Haba
,
Isabelle Kaneza
,
Sookyoung Jeon
,
Eleonora Scorletti
,
Rotonya M. Carr
1
Division of Gastroenterology
,
Perelman School of Medicine, University of Pennsylvania
Chapters
00:04
Title
00:54
Tissue Preparation
03:10
Lipid Droplet (LD) Isolation
05:18
LD Washing
06:38
Results: Analysis of LDs after Isolation
08:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方案建立了一种新的方法, 从小鼠肝脏脂滴分离和纯化, 使用一个完善的内质网隔离试剂盒。
Tags
Lipid Droplet
Organelle Isolation
Liver
Mouse
ER
Lysosome
Homogenization
Centrifugation
IE Buffer
Rapid Isolation
Article
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