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嗜酸性粒植物视网膜免疫组织化学、西方分析和 Rna 分离的解剖
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Developmental Biology
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JoVE Journal Developmental Biology
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
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嗜酸性粒植物视网膜免疫组织化学、西方分析和 Rna 分离的解剖

DOI:
10.3791/59299-v

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08:47 min

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March 15, 2019

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,
1Department of Biology,Wesleyan University

Chapters

  • 00:04Title
  • 00:29Eye-Brain Complex Dissection
  • 02:09Tissue Processing for Immunofluorescence
  • 04:56Tissue Processing for Western Blotting
  • 06:07Tissue Processing for RNA Extraction
  • 07:07Results: Representative Pupal Eye Protein and Gene Expression Analyses
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了一种解剖嗜酸性粒体木偶视网膜的手术方法, 以及免疫组织化学、西方分析和 rna 提取组织处理的方案。

Tags

DrosophilaPupal RetinaImmunohistochemistryWestern AnalysisRNA IsolationDissectionEye-brain ComplexMicrodissectionForcepsPBSFixative

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