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Dissektion der Drosophila Pupal Netzhaut Immunohistochemistry, westlichen Analyse und RNA-Isolation
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
DOI:

08:47 min

March 15, 2019

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Chapters

  • 00:04Title
  • 00:29Eye-Brain Complex Dissection
  • 02:09Tissue Processing for Immunofluorescence
  • 04:56Tissue Processing for Western Blotting
  • 06:07Tissue Processing for RNA Extraction
  • 07:07Results: Representative Pupal Eye Protein and Gene Expression Analyses
  • 08:28Conclusion

Summary

Automatic Translation

Dieses Papier stellt eine Operationsmethode für sezieren Drosophila pupal Netzhaut zusammen mit Protokolle für die Verarbeitung von Gewebe für Immunohistochemistry, westlichen Analyse und RNA-Extraktion.

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