Journal
/
/
免疫組織化学、西部の分析、および RNA の隔離のためのショウジョウバエ蛹網膜の解剖
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
DOI:

08:47 min

March 15, 2019

,

Chapters

  • 00:04Title
  • 00:29Eye-Brain Complex Dissection
  • 02:09Tissue Processing for Immunofluorescence
  • 04:56Tissue Processing for Western Blotting
  • 06:07Tissue Processing for RNA Extraction
  • 07:07Results: Representative Pupal Eye Protein and Gene Expression Analyses
  • 08:28Conclusion

Summary

Automatic Translation

免疫組織化学、西部の分析、および RNA 抽出のための組織の処理のためのプロトコル、ショウジョウバエ蛹網膜を解剖するための手術方法を提案する.

Related Videos

Read Article