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Journal
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Biochemistry
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通过建立单罐双酶级联,从弗拉瓦内生物合成一种黄酮
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Biochemistry
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JoVE Journal
Biochemistry
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Please note that all translations are automatically generated.
Click here for the English version.
通过建立单罐双酶级联,从弗拉瓦内生物合成一种黄酮
DOI:
10.3791/59336-v
•
09:50 min
•
August 14, 2019
•
Zhiping Zhang
,
Shuhang Fan
,
Zhiqiang Chen
,
Yanzhi He
,
Mengfei Huang
,
Li Ding
,
Yanyan Zhang
,
Lin Chen
,
Xinyue Zhang
2,3,4
1
College of Bioscience and Biotechnology
,
Yangzhou University
,
2
Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China
,
Yangzhou University
,
3
Joint International Research Laboratory of Agriculture & Agri-Product Safety
,
Yangzhou University
,
4
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses
,
5
The Testing Center
,
Yangzhou University
Chapters
00:04
Title
01:13
Produce a Flavonol from a Flavanone in an in vitro Bienzyme Synthetic System
02:48
Thin Layer Chromatography (TLC) Analysis
05:21
High Performance Liquid Chromatography (HPLC) and Liquid Chromatography/ Mass Spectrometry (LC/MS) Analyses
07:50
Results: Conversion of Flavanone into Flavono
09:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
黄酮醇的衍生对于其在医疗保健和食品工业中的应用至关重要。在这里,我们为黄酮中黄酮的生物合成提供详细的方案,并讨论与其他方法不同的关键步骤及其优势。
Tags
Flavonol Biosynthesis
Flavanone
One-pot Bienzymatic Cascade
Recombinant Enzymes
Secondary Metabolite Production
Synthetic Buffer
Flavonoid Stock Solution
TLC Analysis
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