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Chapters
- 00:04Title
- 00:34High Precision Coverslip and Glass Slide Preparation
- 01:17Fluorescent Microsphere and Spore Sample Mounting
- 02:32Fluorescent Microsphere and Spore Imaging
- 03:38FM4-64 Stained PS4150 Spore 3D-SIM Raw Image Reconstruction
- 05:00Image Analysis
- 06:25Results: Representative Germinosome and Inner Membrane Visualization
- 08:04Conclusion
Germinant reseptör proteinler 'germinosomes' Bacillus subtilis sporlar iç zarda kümesinde. Biz süper çözünürlük mikroskobu ve floresan muhabir proteinleri germinosomes görselleştirmek için bir iletişim kuralı'nı açıklar. İletişim kuralı da tercihen membran boya FM4-64 ile lekeli spor iç zar etki alanları tanımlar.
Tags
GerminosomesInner MembraneBacillus Subtilis SporesSuper-resolution Structured Illumination Microscopy3D-SIMFluorescent Reporter ProteinsSpore MembranesLipid DomainsCoverslipsMicroscope SlidesFluorescent MicrospheresAgaroseUltrapure WaterGene FrameStructured Illumination MicroscopePoint Spread FunctionExcitation Wavelengths
