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Visualização do Germinosomes e a membrana interna em esporos de Bacillus subtilis
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores
DOI:

08:58 min

April 15, 2019

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Chapters

  • 00:04Title
  • 00:34High Precision Coverslip and Glass Slide Preparation
  • 01:17Fluorescent Microsphere and Spore Sample Mounting
  • 02:32Fluorescent Microsphere and Spore Imaging
  • 03:38FM4-64 Stained PS4150 Spore 3D-SIM Raw Image Reconstruction
  • 05:00Image Analysis
  • 06:25Results: Representative Germinosome and Inner Membrane Visualization
  • 08:04Conclusion

Summary

Automatic Translation

Cluster de proteínas do receptor germinant em 'germinosomes' na membrana interna dos esporos de Bacillus subtilis . Descreveremos um protocolo usando proteínas de repórter de microscopia e fluorescente super resolução para visualizar germinosomes. O protocolo também identifica domínios de membrana interna de esporo que preferencialmente são corados com o corante de membrana FM4-64.

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