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En in vitro modell for å studere tau aggregering bruke Lentiviral-mediert Transduction av Human neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
DOI:

05:51 min

May 23, 2019

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Chapters

  • 00:04Title
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

Automatic Translation

Denne protokollen detaljer en prosedyre der menneskelige neuronal kulturer er transduced med lentiviral konstruerer koding for mutant menneskelig tau. Transduced kulturer vise tau aggregater og tilhørende patologi.

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