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En in vitro modell for å studere tau aggregering bruke Lentiviral-mediert Transduction av Human neurons
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Neuroscience
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JoVE Journal Neuroscience
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

En in vitro modell for å studere tau aggregering bruke Lentiviral-mediert Transduction av Human neurons

DOI:
10.3791/59433-v

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05:51 min

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May 23, 2019

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, , ,
1Department of Neurosciences,University of California, San Diego

Chapters

  • 00:04Title
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen detaljer en prosedyre der menneskelige neuronal kulturer er transduced med lentiviral konstruerer koding for mutant menneskelig tau. Transduced kulturer vise tau aggregater og tilhørende patologi.

Tags

Tau AggregationIn Vitro ModelHuman NeuronsLentiviral TransductionCell CultureDrug ScreeningDisease MechanismBasement Membrane MatrixNeural Stem Cell MediaBFGFDMEM-F12Penicillin-streptomycinCell ThawingCentrifugationCell SeedingConfluencyLentivirus Transduction

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