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通过将表达的腺病毒注射到小鼠乳腺腺中,模拟乳腺癌
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Cancer Research
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JoVE Journal Cancer Research
Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland
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通过将表达的腺病毒注射到小鼠乳腺腺中,模拟乳腺癌

DOI:
10.3791/59502-v

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06:29 min

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June 07, 2019

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, ,
1Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, 2Department of Medicine,Harvard Medical School

Chapters

  • 00:04Title
  • 00:34Preoperative Preparation
  • 01:48Intraductal Injection
  • 03:53Results: Key Features of Genetic Manipulations of Mammary Epithelial Cells Based on Intraductal Injection of Ad-Cre
  • 05:25Conclusion

Summary

Automatic Translation

Automatic Translation

该协议的目的是描述一种新的乳腺癌建模方法,该方法基于将Cre-表达腺病毒注射到小鼠乳腺的电位内。这种方法允许以时间控制的方式对致癌事件进行细胞型和器官特异性操作。

Tags

Breast CancerMouse ModelIntraductal InjectionCre-expressing AdenovirusMammary GlandMammary Epithelial CellsSurgical ProcedureAnesthesiaAnalgesiaHair RemovalDisinfectionIncisionNipple RemovalHamilton SyringeDyeDuctal Tree

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