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Journal
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Developmental Biology
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一种对绿色藻
原
体同步文化进行深入分析的多表率提取方法
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
A Multi-Omics Extraction Method for the In-Depth Analysis of Synchronized Cultures of the Green Alga
Chlamydomonas reinhardtii
Please note that all translations are automatically generated.
Click here for the English version.
一种对绿色藻
原
体同步文化进行深入分析的多表率提取方法
DOI:
10.3791/59547-v
•
07:51 min
•
August 08, 2019
•
Umarah Mubeen
,
Lais Albuquerque Giraldi
,
Jessica Jüppner
,
Patrick Giavalisco
2
1
Max Planck Institute of Molecular Plant Physiology
,
2
Max Planck Institute of Biology of Ageing
Chapters
00:04
Title
00:32
Chlorophyll, Lipid, and Metabolite Extraction and Fraction Allocation
02:04
Polar (Primary) and Non-Polar (Lipid) Metabolite and Chlorophyll Content Determination
03:35
Extraction and Determination of Protein Content, Digestion, and Analysis
05:53
Results: Representative Synchronized Culture Analyses
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
对多个生物分子进行系统分析对于获得对生物过程的功能和机械见解至关重要。因此,描述了一个广泛的协议,用于从从同步衣原体培养中采集的单一样本中高通量提取脂质、代谢物、蛋白质和淀粉。
Tags
Multi-omics
Chlamydomonas Reinhardtii
Chlorophyll
Lipids
Metabolites
Proteins
Starch
Extraction
Gas Chromatography-mass Spectrometry
Liquid Chromatography-mass Spectrometry
Synchronized Cultures
Systems Biology
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