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定量细菌中蛋白质-RNA结合的测定

JoVE Journal
Genetics

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JoVE Journal Genetics

An Assay for Quantifying Protein-RNA Binding in Bacteria

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定量细菌中蛋白质-RNA结合的测定

DOI:

10.3791/59611-v

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07:02 min

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June 12, 2019

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Noa Katz*¹, Roni Cohen*¹, Orna Atar, Sarah Goldberg, Roee Amit²

¹Department of Biotechnology and Food Engineering,Technion-Israel Institute of Technology, ²Russell Berrie Nanotechnology Institute,Technion-Israel Institute of Technology

Chapters

00:04Title
00:44Design of Binding-site Plasmids
02:12Design and Construction of the RBP Plasmid
03:11Experiment Setup
04:56Results: Quantifying Protein-RNA Binding in Bacteria
06:32Conclusion

Summary

Automatic Translation

在这种方法中,我们使用在细菌细胞中的简单、实时的、报告学测定来量化RNA结合蛋白(RBPs)与共和结合位点的结合亲和力。测定基于对报告者基因的抑制。

Tags

Protein-RNA Binding RNA Binding Protein RBP-RNA Affinity In Vivo Plasmid Design Binding Site Cassette MCherry Reporter Gene RBP Sequence Bacterial Transformation Glycerol Stocks

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