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Chapters
- 00:04Title
- 01:14Tissue Harvesting and UV Crosslinking
- 02:37Beads Preparation
- 03:20Tissue Lysis and Partial RNA Digestion
- 04:30Immunoprecipitation
- 05:09RNA Adapter Ligation to RNA 3’ Ends
- 06:48SDS-PAGE and Membrane Transfer
- 08:24Results: Analysis of the eCLIP Method
- 10:08Conclusion
