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新生大鼠脑组织在神经端子突形圆锥花序分布超微结构形态分析中的制备
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
Please note that all translations are automatically generated.
Click here for the English version.
新生大鼠脑组织在神经端子突形圆锥花序分布超微结构形态分析中的制备
DOI:
10.3791/59694-v
•
10:09 min
•
June 07, 2019
•
Bianca Ferrarese
2
,
Nadia Lunardi
1
Department of Anesthesiology
,
University of Virginia Health System
,
2
Department of Anesthesiology
,
Universita’ degli Studi di Padova
Chapters
00:04
Title
01:27
Post-fixation, Sequential Dehydration, and Staining of Brain Sections
03:38
Infiltration and Embedding
05:01
Flat Embedding
06:09
Capsule Embedding
07:28
Results: Electron Micrographs of Newborn Rat Brain Tissue
09:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了处理新生大鼠脑组织以获得高分辨率的电子显微镜的程序, 以进行神经末端突触囊分布的形态分析。用这些方法获得的显微图像也可用于研究许多其他细胞成分的形态及其尺寸结构关系。
Tags
Newborn Rat Brain
Ultrastructural Morphometric Analysis
Synaptic Vesicle Distribution
Nerve Terminals
Transmission Electron Microscopy
Osmium Tetroxide
Phosphate Buffer
Uranyl Acetate
Synaptic Function
Synaptic Development
Anesthetics
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