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新生大鼠脑组织在神经端子突形圆锥花序分布超微结构形态分析中的制备
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
DOI:

10:09 min

June 07, 2019

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Chapters

  • 00:04Title
  • 01:27Post-fixation, Sequential Dehydration, and Staining of Brain Sections
  • 03:38Infiltration and Embedding
  • 05:01Flat Embedding
  • 06:09Capsule Embedding
  • 07:28Results: Electron Micrographs of Newborn Rat Brain Tissue
  • 09:02Conclusion

Summary

Automatic Translation

我们描述了处理新生大鼠脑组织以获得高分辨率的电子显微镜的程序, 以进行神经末端突触囊分布的形态分析。用这些方法获得的显微图像也可用于研究许多其他细胞成分的形态及其尺寸结构关系。

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