Journal
/
Developmental Biology
/
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

DOI:
10.3791/59743-v

•

08:36 min

•

July 16, 2019

•

, , , , , ,
1Department of Plant Science and Landscape Architecture,University of Maryland, College Park

Chapters

  • 00:04Title
  • 00:43Preparation of Dosage Curve for Effective Mutagenesis
  • 03:09Cel-1 Assay for Genetic Characterization of Mutants
  • 06:03Results: EMS Mutation
  • 07:37Conclusion

Summary

Automatic Translation

Automatic Translation

Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.

Tags

Keywords: TILLINGMutagenesisEMSSmall Grain CropsFunctional GenomicsGene DiscoveryCel-1 AssayMutation DetectionDosage OptimizationM2 Plants

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59743?article_type=v

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury
check_url/59743?article_type=v

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
check_url/59743?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/59743?article_type=v

Technique to Target Microinjection to the Developing Xenopus Kidney
check_url/59743?article_type=v

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
check_url/59743?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/59743?article_type=v

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
check_url/59743?article_type=v

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans
check_url/59743?article_type=v

Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
check_url/59743?article_type=v

Controlled Cortical Impact Model of Mouse Brain Injury with Therapeutic Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Cells

Read Article