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JoVE Journal Developmental Biology
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
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Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Article DOI: 10.3791/59743-v • 08:36 min • July 16th, 2019
July 16th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park

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Summary
Automatic Translation

Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.

Tags

TILLING Small Grain Crops EMS Mutagenesis Mutation Frequency Functional Genomics Forward Genetic Space Gene Discovery Cel-1 Assay EMS Concentration Genotype Imbibition Fume Hood EMS Solution
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