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通过乙基甲烷硫素突变在小谷物作物中靶向诱导局部病变(TILLING)种群的发展

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Developmental Biology

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JoVE Journal Developmental Biology

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

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通过乙基甲烷硫素突变在小谷物作物中靶向诱导局部病变(TILLING)种群的发展

DOI:

10.3791/59743-v

•

08:36 min

•

July 16, 2019

•

Lovepreet Singh, Adam Schoen, Alexander Mahlandt, Bhavit Chhabra, James Steadham, Vijay Tiwari, Nidhi Rawat

¹Department of Plant Science and Landscape Architecture,University of Maryland, College Park

Chapters

00:04Title
00:43Preparation of Dosage Curve for Effective Mutagenesis
03:09Cel-1 Assay for Genetic Characterization of Mutants
06:03Results: EMS Mutation
07:37Conclusion

Summary

Automatic Translation

描述是使用乙基甲酸酯 (EMS) 作为诱变剂在小谷物作物中开发靶向诱导局部病变(TILLING)人群的协议。还提供了使用Cel-1测定突变检测的协议。

Tags

Keywords: TILLING Mutagenesis EMS Small Grain Crops Functional Genomics Gene Discovery Cel-1 Assay Mutation Detection Dosage Optimization M2 Plants

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