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Developmental Biology

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JoVE Journal Developmental Biology
Utveckling av riktade inducerade lokala lesioner i genomer (TILLING) populationer i små spannmålsgrödor med Etylmetanesulfonatmuta Genes
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Utveckling av riktade inducerade lokala lesioner i genomer (TILLING) populationer i små spannmålsgrödor med Etylmetanesulfonatmuta Genes

Article DOI: 10.3791/59743-v • 08:36 min • July 16th, 2019
July 16th, 2019 •
1, 1, 1, 1, 1, 1, 1
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park

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Beskrivs är ett protokoll för att utveckla en inriktning inducerade lokala lesioner i Genomes (TILLING) population i små spannmålsgrödor med användning av etylmetanesulfonat (EMS) som mutagen. Det finns också ett protokoll för Mutations detektering med hjälp av cel-1-analysen.

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Utvecklingsbiologi utgåva 149 mutagenes TILLING population EMS omvänd genetik små korn cel-1 Endonuklease
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