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Immunology and Infection
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在定义良好的剪切应力下单细胞电电流的电生理记录
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Immunology and Infection
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Immunology and Infection
Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress
Please note that all translations are automatically generated.
Click here for the English version.
在定义良好的剪切应力下单细胞电电流的电生理记录
DOI:
10.3791/59776-v
•
07:17 min
•
August 02, 2019
•
Ibra S. Fancher
,
Irena Levitan
1
Department of Medicine, Division of Pulmonary, Critical Care, Sleep and Allergy
,
University of Illinois at Chicago
Chapters
00:04
Title
00:29
Assembly of the Modified Parallel Plate Flow Chamber
01:43
Cell Preparation and Seeding into the MPP Flow Chamber
03:43
Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
05:46
Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
06:43
Conclusion
Summary
Automatic Translation
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该协议的目的是描述一个经过修改的平行板流室,用于研究通过剪切应力实时激活美能敏感子孔通道。
Tags
Electrophysiological Recordings
Single-cell Ion Currents
Shear Stress
Parallel Plate Flow Chamber
Mechanosensitive Ion Channels
Silicone Elastomer
Cover Glass
Cell Culture
Electrophysiological BAF Solution
Cell Adhesion
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