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Elektrofysiologiska inspelningar av encellig Jon strömmar under väldefinierad skjuvning stress
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress
DOI:

07:17 min

August 02, 2019

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Chapters

  • 00:04Title
  • 00:29Assembly of the Modified Parallel Plate Flow Chamber
  • 01:43Cell Preparation and Seeding into the MPP Flow Chamber
  • 03:43Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
  • 05:46Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
  • 06:43Conclusion

Summary

Automatic Translation

Målet med detta protokoll är att beskriva en modifierad parallell platta flödes kammare för användning vid utredning av realtids aktivering av mechanosensitive jonkanaler genom skjuvning stress.

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