Journal
/
Immunology and Infection
/
Purification of a High Molecular Mass Protein in Streptococcus mutans
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Purification of a High Molecular Mass Protein in Streptococcus mutans

Purification of a High Molecular Mass Protein in Streptococcus mutans

DOI:
10.3791/59804-v

•

09:51 min

•

September 14, 2019

•

, , , ,
1Department of Translational Research,Tsurumi University School of Dental Medicine, 2Laboratory for Oral Health Science

Chapters

  • 00:06Title
  • 00:51PCR Amplification
  • 02:38Cell Transformation
  • 03:35Purification of Polyhistidine-tagged GTF-SI
  • 07:29Results: Generation of S. mutans His-gtfC and Functional Restoration
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.

Tags

Keywords: Protein PurificationStreptococcus MutansHigh Molecular Mass ProteinPCRGenomic DNA ExtractionGel ElectrophoresisGel ExtractionNested PCRElectroporationCompetent CellsBrain-heart Infusion Broth

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59804?article_type=v

Using Bioluminescent Imaging to Investigate Synergism Between Streptococcus pneumoniae and Influenza A Virus in Infant Mice
check_url/59804?article_type=v

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging
check_url/59804?article_type=v

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
check_url/59804?article_type=v

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
check_url/59804?article_type=v

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
check_url/59804?article_type=v

High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
check_url/59804?article_type=v

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
check_url/59804?article_type=v

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
check_url/59804?article_type=v

RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
check_url/59804?article_type=v

A Murine Model of Group B Streptococcus Vaginal Colonization

Read Article