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Analyse des Lipidtröpfchengehalts in Spalt- und Budding-Hefen mittels automatisierter Bildverarbeitung
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing
DOI:

08:43 min

July 17, 2019

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Chapters

  • 00:04Title
  • 00:31Lipid Droplet Staining
  • 02:39Imaging Setup and Acquisition
  • 04:40Image Analysis: Quality Check
  • 05:39Image Analysis: MATLAB
  • 06:27Results: Changes in Lipid Droplet Number, Size, and Lipid Content in Three Morphologically Distinct Yeast Species
  • 08:06Conclusion

Summary

Automatic Translation

Hier stellen wir eine MATLAB-Implementierung des automatisierten Nachweises und der quantitativen Beschreibung von Lipidtröpfchen in Fluoreszenzmikroskopiebildern von Spalt- und angehenden Hefezellen vor.

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