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La detección de 5-Hidroximetilcitosina en células madre neuronales y cerebros de ratones
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice
DOI:

08:03 min

September 19, 2019

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Chapters

  • 00:04Title
  • 01:00Transcardial Perfusion of the Mouse
  • 02:03Brain Sectioning and Immunofluorescence Staining
  • 04:02Genomic DNA Isolation
  • 05:43DNA Dot Blot
  • 07:03Results: Immunofluorescence Staining and Dot Blot Detection
  • 07:49Conclusion

Summary

Automatic Translation

Aquí, presentamos un protocolo para detectar 5-hidroximetilcitosina en células y tejidos cerebrales, utilizando la tinción de inmunofluorescencia y métodos de adn-blot.

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