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Neuroscience
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小鼠神经干细胞和脑中5-羟基甲基细胞氨酸的检测
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice
Please note that all translations are automatically generated.
Click here for the English version.
小鼠神经干细胞和脑中5-羟基甲基细胞氨酸的检测
DOI:
10.3791/59950-v
•
08:03 min
•
September 19, 2019
•
Yingliang Zhuang
2
,
Junchen Chen
2
,
Weize Xu
,
Qiang Shu
,
Xuekun Li
2
1
The Children’s Hospital, School of Medicine
,
Zhejiang University
,
2
The Institute of Translational Medicine, School of Medicine
,
Zhejiang University
Chapters
00:04
Title
01:00
Transcardial Perfusion of the Mouse
02:03
Brain Sectioning and Immunofluorescence Staining
04:02
Genomic DNA Isolation
05:43
DNA Dot Blot
07:03
Results: Immunofluorescence Staining and Dot Blot Detection
07:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一个协议,利用免疫荧光染色和DNA点印法检测细胞和脑组织中5-羟基甲基细胞氨酸。
Tags
5-hydroxymethylcytosine
5hmC
Immunofluorescence
DNA Dot-blot
Neural Stem Cells
Mouse Brain
Epigenetic Modification
Gene Expression
Neurological Disorders
Perfusion
Fixation
Cryosectioning
Immunostaining
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