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Mikrobiota-analyse ved hjælp af to-trins PCR og næste generations 16S rRNA-Gensekvensering
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Mikrobiota-analyse ved hjælp af to-trins PCR og næste generations 16S rRNA-Gensekvensering

Article DOI: 10.3791/59980-v • 11:22 min • October 15th, 2019
October 15th, 2019 •
1, 2, 1, 1,2,3,4
1Department of Pathology, University of Iowa, 2Medical Scientist Training Program, University of Iowa, 3Graduate Program in Immunology, University of Iowa, 4Graduate Program in Molecular Medicine, University of Iowa

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Beskrevet her er en forenklet standard betjeningsprocedure for mikrobiome profilering ved hjælp af 16S rRNA metagenomanalyse sekvensering og analyse ved hjælp af frit tilgængelige værktøjer. Denne protokol vil hjælpe forskere, som er nye inden for mikrobiom-området, samt dem, der kræver opdateringer om metoder til at opnå bakteriel profilering ved en højere opløsning.

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Biologi fecal DNA ekstraktion bibliotek forberedelse 16s variabel region 16S rRNA næste generation Sequencing Gut mikrobiota
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