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使用两步PCR和下一代16S rRNA基因测序进行微生物群分析
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
使用两步PCR和下一代16S rRNA基因测序进行微生物群分析
DOI:
10.3791/59980-v
•
11:22 min
•
October 15, 2019
•
Shailesh K. Shahi
,
Kasra Zarei
,
Natalya V. Guseva
,
Ashutosh K. Mangalam
2,3,4
1
Department of Pathology
,
University of Iowa
,
2
Medical Scientist Training Program
,
University of Iowa
,
3
Graduate Program in Immunology
,
University of Iowa
,
4
Graduate Program in Molecular Medicine
,
University of Iowa
Chapters
00:04
Title
01:25
Extraction of DNA from Fecal Sample
02:29
16S rRNA Gene Amplification (PCR1) and DNA Library Construction (PCR2)
03:57
Clean-up of Indexed PCR (PCR2) and Quantification
06:03
Sequence Analysis
07:49
Results: Quality Profile and Statistical Analysis
09:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里描述的是使用16S rRNA基因组测序和利用免费工具进行分析的微生物群分析的简化标准操作程序。该协议将帮助那些对微生物群领域较新的研究人员,以及那些需要更新方法以更高分辨率实现细菌分析的研究人员。
Tags
Microbiome
Microbiota
16S RRNA Gene Sequencing
Next-generation Sequencing
Fecal Sample
DNA Extraction
PCR
Gut Microbiome
Microbial Profiling
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