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Journal
/
Biochemistry
/
大肠杆菌
中异构表达对膜迁移者的特性与膜囊泡的产生
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Characterization of Membrane Transporters by Heterologous Expression in
E. coli
and Production of Membrane Vesicles
Please note that all translations are automatically generated.
Click here for the English version.
大肠杆菌
中异构表达对膜迁移者的特性与膜囊泡的产生
DOI:
10.3791/60009-v
•
13:16 min
•
December 31, 2019
•
Menaka Ariyaratne
,
Lingxiao Ge
,
Paul F. Morris
1
Department of Biological Sciences
,
Bowling Green State University
Chapters
00:05
Title
01:18
Generation of Inside-out Membrane Vesicles
04:21
Western Blot and Orientation of Transporter Assay
06:46
Transport Assay
08:44
Results: Characterization of Membrane Transporters
11:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种在
大肠杆菌
中的异质表达和利用法国印刷机对细胞进行分理的膜囊泡制剂中质子驱动膜输送剂的表征方法。
Tags
Membrane Transporters
Heterologous Expression
E. Coli
Membrane Vesicles
Protein Gradients
Substrate Transport
Affinity
Competition Assays
Substrate Activation
French Press
Ultracentrifugation
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