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Vurdering af lymfocyt migration i et ex vivo-Transmigrerings system
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
DOI:

10:25 min

September 20, 2019

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Chapters

  • 00:04Title
  • 00:50Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
  • 04:25Ex vivo Transmigration Procedure
  • 06:39Quantification of Ex vivo Transmigration
  • 08:06Results: Assessment of Lymphocyte Migration under Various Conditions
  • 09:42Conclusion

Summary

Automatic Translation

I denne protokol er lymfocytter placeret i det øverste kammer af et transmigrerings system, adskilt fra det nederste kammer af en porøs membran. Chemokin tilsættes til det nederste kammer, som inducerer aktiv migration langs en chemokine gradient. Efter 48 h tælles lymfocytter i begge kamre for at kvantificere overførsel.

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