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Vurdering af lymfocyt migration i et ex vivo-Transmigrerings system
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Immunology and Infection
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JoVE Journal Immunology and Infection
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Vurdering af lymfocyt migration i et ex vivo-Transmigrerings system

DOI:
10.3791/60060-v

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10:25 min

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September 20, 2019

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,
1Department of Internal Medicine,University of Utah, 2Research Service,VA Nebraska Iowa Health Care System, 3Department of Internal Medicine,University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:50Isolation of CD4+ T Cells from Spleens and Lungs of OVA-challenged Mice
  • 04:25Ex vivo Transmigration Procedure
  • 06:39Quantification of Ex vivo Transmigration
  • 08:06Results: Assessment of Lymphocyte Migration under Various Conditions
  • 09:42Conclusion

Summary

Automatic Translation

Automatic Translation

I denne protokol er lymfocytter placeret i det øverste kammer af et transmigrerings system, adskilt fra det nederste kammer af en porøs membran. Chemokin tilsættes til det nederste kammer, som inducerer aktiv migration langs en chemokine gradient. Efter 48 h tælles lymfocytter i begge kamre for at kvantificere overførsel.

Tags

Lymphocyte MigrationTransmigrationImmune ResponseChemokinesILC2Ex VivoCell IsolationCell CountingCell Suspension

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