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Genetics
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在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰
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Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰
DOI:
10.3791/60085-v
•
09:04 min
•
September 25, 2019
•
Fabian L. Cardenas-Diaz*
1
,
Jean Ann Maguire*
1
,
Paul Gadue
2,3
,
Deborah L. French
2,3
1
Center for Cellular and Molecular Therapeutics
,
The Children’s Hospital of Philadelphia
,
2
Department of Pathology and Laboratory Medicine
,
The Children’s Hospital of Philadelphia
,
3
Department of Pathology and Laboratory Medicine
,
University of Pennsylvania
Chapters
00:04
Title
01:19
Test gRNA (GuidE RNA) Cutting Efficiency
03:50
Clone Screening
04:51
Transfection Setup
06:07
Checking for Mutations in Single Colonies
07:11
Results: Cutting Efficiency, and Generation and Screening of Mutations
08:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提供了一种方法,促进在人类多能干细胞中使用CRISPR-CAS9生成定义的异质核苷酸或同源核苷酸变化。
Tags
CRISPR-Cas9
Human Pluripotent Stem Cells
Isogenic Lines
Heterozygous Mutations
Gene Function
Disease Modeling
Colony Picking
Fluorescence-activated Cell Sorting
MEFs
ROCK Inhibitor
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