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Journal
/
Neuroscience
/
从胚胎小鼠小脑分离的神经元的主要培养
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
Please note that all translations are automatically generated.
Click here for the English version.
从胚胎小鼠小脑分离的神经元的主要培养
DOI:
10.3791/60168-v
•
08:09 min
•
October 26, 2019
•
Shahin Shabanipour
2
,
Azadeh Dalvand
2
,
Xiaodan Jiao
2
,
Maryam Rahimi Balaei
2
,
Seung H. Chung
,
Jiming Kong
,
Marc. R. Del Bigio
4
,
Hassan Marzban
2
1
Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences
,
University of Manitoba
,
2
The Children’s Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences
,
University of Manitoba
,
3
Department of Oral Biology
,
University of Illinois at Chicago
,
4
Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences
,
University of Manitoba
Chapters
00:00
Title
00:29
Protocol
06:32
Results
07:41
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
进行体外实验以尽可能充分地反映体内状况并非易事。使用原发细胞培养物是了解整个生物体中细胞生物学的重要一步。提供的协议概述了如何成功生长和培养胚胎小鼠小脑神经元。
Tags
Primary Neuronal Cell Culture
Embryonic Mouse Cerebellum
In Vitro
Dissociated Neurons
Cellular Features
Mechanisms
Functions
Morphology
Poly-L-ornithine
Trypsin
DMEM/F12
HBSS
PBS
Dissection Medium
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