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从患者前列腺癌骨转移标本及其异种移植物中提取的三维(3D)有机物的建立与分析
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Establishment and Analysis of Three-Dimensional (3D) Organoids Derived from Patient Prostate Cancer Bone Metastasis Specimens and their Xenografts
DOI:

07:21 min

February 03, 2020

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Chapters

  • 00:05Introduction
  • 01:24Forming an Attached Round Dome
  • 02:23Forming Floating Beads
  • 03:19Organoid Processing for Histology
  • 05:24Results: 3D Organoids from Patient-derived Xenograft and Primary Tumor Tissue
  • 06:17Conclusion

Summary

Automatic Translation

患者BMPC标本和骨转移性前列腺癌异种移植物的三维培养,保持其原始肿瘤的功能异质性,导致囊肿、球形和复杂的肿瘤状有机体。本手稿为异质患者衍生样本的 3D 培养及其使用 IFC 进行分析提供了优化策略和方案。

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