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Kanser Tedavisi için Sitotoksik Sitokin Kaynaklı Katil T Hücrelerinin İzolasyon ve Genişlemesi
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1Department of Urology,Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine,Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine,Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research,China Medical University Hospital, 5Graduate Institute of Biomedical Sciences,China Medical University, 6Graduate Institute of Immunology,China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center,China Medical University Hospital
Chapters
- 00:04Introduction
- 00:50Immunophenotyping for Assessment of CIK Cells
- 02:14CD Marker Recognition
- 04:12Cytotoxic Assay
- 08:15Results: Peripheral Blood Mononuclear Cell (PBMC)-Derived CIK Expansion and Characterization and the Cytotoxicity Assay
- 10:10Conclusion
Burada periferik kan mononükleer hücrelerinin izolasyon ve genleşmesini gerçekleştirmek için bir protokol sunmaktayız- periferik kan mononükleer hücrelerden türetilmiş sitokin kaynaklı CD3+CD56+ öldürücü hücreler ve in vitro tanı akış sitometri sistemi kullanarak hematolojik ve katı kanser hücrelerine karşı sitotoksisite etkilerini göstermek.
